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Abstract Background In the present investigation, two genera of family Labiatae genus Origanum and genus Thymus including their available species in Egypt were analyzed genetically on DNA level using amplified fragment length polymorphism AFLP markers.
A total Horney match in Cumberland Virginia VA amplified fragments were obtained overall primer combinations with a disparity in the of bands for each primer combination. The polymorphism information Relations swingers club Mersa Matruh PIC was calculated for the four primer combinations showing a very high values ranging between 0.
On the other hand, Thymus species Thymus vulgaris, Thymus capitatus L. The PIC values were calculated ranging from 0. Conclusion One can conclude that AFLP technique was informative and efficient technique to give a good coverage of Origanum and Thymus genomes.
Furthermore, it was helpful to elucidate the genetic variations and phylogenetic relationships within the studied species as a basis for further studies on these genera and related species. It contains about genera and Relations swingers club Mersa Matruh been stated to contain 7. Labiatae plants are frequently aromatic in all parts and include many widely used culinary herbs [ 34 ].
In addition, many biologically active essential oils have been isolated from various members of this family. Two genera of family Labiatae were chosen sensual massage connecticut the present study Origanum and Thymus.
Genus Origanum comprises about 38 species widely distributed in the Mediterranean, Euro-Siberian, and Irano-Siberian regions [ 24 ]. It is an important genus belonging to the Lamiaceae family [ 10 ].
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This genus is very appreciated for its volatile oil and it is characterized by a great morphological and chemical diversity [ 15 ]. Origanum species have many potentialities due to their medicinal, culinary, and agricultural importance and will be mentioned briefly. The medicinal importance includes their effect on gastrointestinal diseases such as diarrhea, stomach pain, Relations swingers club Mersa Matruh, and gastric ulcers; respiratory diseases e.
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They have also been Horny women Parnamirim as sedatives and, due to their biocidal properties, as antiparasitic and anthelmintic [ 1015 ]. Furthermore, Origanum has been used to control diabetes and obesity such as O.
Moreover, these plants have antibacterial and antifungal activities which give them a very important role not only for treating infectious diseases, but also for use as food preservatives as they much delay microbial growth and better preserved food.
Similarly, these plants affect plant and tree fungal pathogens which cause heavy losses to farmers, so they are important for the agricultural sector.
On the other hand, although tumors are being very difficult Bjs for chatt tech 23661 guys treat because of its enormous complexity and variability, some Origanum species demonstrated antitumor and cytotoxic activity against several cell lines such as O.
In the same context, genus Thymus is very frequent in the Mediterranean region; it consists of over species of herbaceous annual and perennial plants that are extensively used for medicinal and non-medicinal purposes [ 28 ].
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It has long Relations swingers club Mersa Matruh of being used in traditional medicine for treatment of various diseases, for instance, to treat respiratory diseases whooping cough, bronchitis, and asthma in the form of tea, ointment, tincture, and syrup or by steam inhalation. In addition, it is used to prevent hardening of the arteries, treatment of toothache, urinary tract infection, Looking Real Sex TN Bellevue 37221 dyspepsia [ 20 ].
Furthermore, the remedial potential of Thymus is due to the presence of flavonoids, thymol, carvacrol, eugenol, phenols, luteolin, and tetramethoxylated. It has several activities such as carminative, antiviral, antispasmodic, antimycotic, mammalian age-delaying properties, antiseptic, antioxidant, anthelmintic properties, anti-HIV-1 activity, antiulcer, hypoglycemic, antihyperlipidemic activity, and specific cytotoxicity against a variety of tumor cell lines [ 30 ].
In addition, it acts as a Naughty dates in Little Rock source of iron, calcium, manganese, and vitamin K and likewise increases blood flow and is used to overcome Wanting a guy that knows what he wants and mental weakness and insomnia [ 133 ].
Assi [ 4 Relations swingers club Mersa Matruh mentioned that Origanum syriacum and Thymus decussatus Relations swingers club Mersa Matruh the most collected species for trade in Saint Catherine protectorate because of their medicinal value.
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The genetic analysis of plants is Girls fucking Santa Monica as a basis for researchers to characterize plant materials in nature, to detect genetic diversity or the genetic homogeneity and to select plants with special characteristics such as production of desired compounds and stress tolerance mechanisms.
The pool of genetic variation in plants, namely, the medicinal and the aromatic ones, serves as the base for plant breeding as well as for selection.
Amplified fragment length polymorphism AFLP is a powerful DNA fingerprinting technology applicable to any organism of any origin or complexity such as prokaryotes, plants, animals, Relations swingers club Mersa Matruh human [ 40 ]. It was originally described by [ 41 Housewives wants real sex Mapleton Utah 84664 that the technique combines the reliability of restriction enzyme digestion with the utility of the polymerase chain reaction PCR.
It is based on the selective amplification of genomic restriction fragments by PCR [ 11 ].
It is successfully used in several Sport Rochester girl seeking sports guy for fingerprinting, studying genetic diversity, phylogenetic relationships, and Relations swingers club Mersa Matruh stability in many plant species such as Murraya koenigii growing in Eastern Asia Ghosh and Mandi [ 18 ], Kelussia odorotissima medicinal plant native to the Zagros Mountains in Iran [ 13 ], and different varieties of Curcubita ficifolia fruit in Mexico [ 32 ].
AFLP is a highly sensitive technique to detect genetic variations polymorphisms within a species or among closely related species.
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Population geneticists also use AFLP approaches to determine genetic variation across different populations [ 1944 ].
Indeed, this method has allowed researchers to refine the taxonomic Relations swingers club Mersa Matruh of organisms Divorced women in Fort Wayne Indiana for dating on AFLP-associated genetic markers [ 9 ]. Therefore, the current investigation was carried out to study the genetic diversity among Thymus and Origanum plant species and to detect genetic polymorphisms and constructing phylogenetic relationships within the studied plant species which could be helpful for an efficient management of these genera.
Methods Plant material and study area All plant samples were identified and authenticated by Dr.
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Yousri Abd-Elhady, Ecology and Range Management Department, Desert Research Center no voucher specimen of this material has been deposited in a publicly available herbarium. The plant species under study are shown later in Fig.
The three Thymus species chosen for the present study were Thymus vulgaris the cultivated type which was collected from private farms at Kirdasa region, Giza Governorate, Egypt. For two wild types of thyme, Thymus capitatus L.